AFGF/FGF1 (Acidic Fibroblast Growth Factor 1), Human, ELISA Kit

Description

AFGF/FGF1 (Acidic Fibroblast Growth Factor 1), Human, ELISA Kit

The kit is for the quantitative level of FGF-1 in the sample, adopt purified Human FGF-1 to coat microtiter plate, make
solid-phase antibody, then add FGF-1 to wells, Combine FGF-1 antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of FGF-1 in the samples is then determined by comparing the O.D. of the samples
to the standard curve.