Mouse pituitary adenylate cyclase activating polypeptide,PACAP ELISAkit

Description

Mouse pituitary adenylate cyclase activating polypeptide,PACAP ELISAkit

The kit is for the quantitative level of PACAP in the sample, adopt purified Rat PACAP to coat microtiter plate, make
solid-phase antibody, then add PACAP to wells, Combine PACAP antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB
substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution
and the color change is measured at a wavelength of 450 nm. The concentration of PACAP in the samples is then
determined by comparing the O.D. of the samples to the standard curve.